Indiscriminate over exploitation from natural source to meet the growing demand by industry coupled with low seed viability, lack of vegetative propagation methods and insufficient attempts for replenishment of wild stock of different important plant species

of vegetative propagation methods and insufficient attempts for replenishment of wild stock of different important plant species have contributed to its threatened status. So realizing the threat of extinction and to meet the growing needs, the alternate method for propagation is micropropagation. This technique is used not only for those plants which are difficult to be propagated through conventional practices, but also for the mass multiplication of existing stocks of germplasm for biomass production and conservation of important, elite and rare plant species that are threatened or on the verge of extinction [1]. In all reports, protocols described are based on conventional micropropagation system (CMS). This CMS explained most common methods of micropropagation (Table 1), which involve initiation/bud proliferation, the shoot multiplication followed by rooting via a semi solid medium. Although CMS have been highly successful in terms of multiplication yields, it has become increasingly important to improve productivity and uniformity of commercially important material in reduced cost, time and space [2]. Hence an improvement in plant tissue culture techniques for the mass propagation of commercial plants is highly desirable [3]. An alternative that has shown potential to improve the efficiency of protocols for in vitro multiplication is the use of a double-phasic culture system (DPS). In this method, the explants remain in the semi-solid medium and the liquid medium is added over periodically throughout the culture, thus A Double Phase Culture System: An Economic and Time Saving Protocol for In Vitro Propagation of Plant Senapati SK*